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xenopus laevis xenopus tropicalis

Experiments were repeated three times. Axeldb A database focussing on gene expression in the frog Xenopus laevis. Kalt MR (1976) Morphology and kinetics of spermatogenesis in Xenopus laevis. Exhibits a high amplitude circadian rhythm with maximal levels in early evening (PubMed:12573214). Xenopus tropicalis and Xenopus laevis environmental parameter standard operating procedure (Kroll lab) From Xenbase. The two best-known species of this genus are Xenopus laevis and Xenopus tropicalis, which are studied as model organisms for developmental biology, cell biology, toxicology, neuroscience and for modelling human disease and birth defects. and Dr. Igawa (Hiroshima Univ. Xenopus contains at least three sarcomeric alpha actin genes that are preferentially expressed in either heart or skeletal muscle. Probable transcription factor. X. laevis frogs are larger than X. tropicalis frogs and so are their cells, eggs, DNA content, and nuclei. Xenopus egg extract is a lysate that is prepared by crushing the eggs of the African clawed frog Xenopus laevis.It offers a powerful cell-free (or in vitro) system for studying various cell biological processes, including cell cycle progression, nuclear transport, DNA replication and chromosome segregation.It is also called Xenopus egg cell-free system or Xenopus egg cell-free extract. 26. Available as frozen sperm, please allow 8 months for live animals. 1. Function i. It was produced by Dr. Fukui (Faculty of Science and Engineering, Chuo Univ.) However, another species of frog Xenopus tropicalis can also be a source. Because X. tropicalis is diploid and X. laevis is tetraploid, important inferences can be made about genome evolution. Xenopus laevis and Xenopus tropicalis have long been used to drive discovery in developmental, cell, and molecular biology. Pattern and morphogenesis of presumptive superficial mesoderm in two closely related species, Xenopus laevis and Xenopus tropicalis. The rapid growth of the National Xenopus Resource has required effective implementation and optimization of these protocols. Xenopus tropicalis Taxonomy ID: 8364 (for references in articles please use NCBI:txid8364) current name. Digitized images and developmental data from Nieuwkoop and Faber (1994) Normal Table of Xenopus laevis (Daudin). Reviewed-Annotation score: -Experimental evidence at transcript level i. Xenopus tropicalis (Gray, 1864) homotypic synonym: Xenopus (Silurana) tropicalis. It has a voracious appetite and attacks anything that passes in front of it. drs6j@virginia.edu The mesoderm, comprising the tissues that come to lie entirely in the deep layer, … Life cycle, evolutionary position and regenerative potential of Xenopus. Room temperature (controlled automatically by building … This is the first amphibian genome to be sequenced, enabling new insights into vertebrate evolution and the last common ancestor of tetrapods. Xenopus laevis and Xenopus tropicalis have been used extensively in developmental and cell biology, biochemistry, functional genomics and immunology. Xenopus tropicalis (Gray, 1864) basionym: Silurana tropicalis Gray, 1864. homotypic synonym: Xenopus (Silurana) tropicalis. Genetic analysis may be applicable to Xenopus (Silurana) tropicalis, which has a diploid genome and a s … Shows a small preference for NADPH over NADP(+) (By similarity). Xenopus laevis (also called the African Clawed frog due to the sharp, black claws in the hind inner three toes) is an aquatic amphibian of the Order Anuran and the family Pipidae.Xenopus are a long-lived, aquatic amphibian commonly used in teaching classrooms and in research laboratories. Carruthersa, S., et. The frog Xenopus laevis has provided significant insights into developmental and cellular processes. tropicalis shares advantages of X. laevis as a classic embryologic system, but its simpler genome and shorter generation time make it more convenient for multigenerational genetic, genomic, and transgenic … (2016) have sequenced the genome of Xenopus laevis. Likewise, X. tropicalis has been selected over X. laevis as the subject of a complete genome sequencing project for the same reason. Genetic Map of Xenopus tropicalis Jamie Davies The kidney development database. If the water temperature is sub optimal... frogs will not eat and their metabolism and immune systems will be depressed. Xenopus tropicalis, on the other hand, possesses a diploid genome and a short generation time, ... (C and D) in stage 55 Xenopus laevis and Xenopus tropicalis tadpoles. Like Xenopus laevis, X. tropicalis was formerly used in pregnancy tests (Bergerard & Lamotte 1949). amphibians, which include Xenopus laevis (X. laevis) and Xenopus tropicalis (X. tropicalis), are one such group of animals with high regenerative capacity (Dinsmore, 1991). A transgenic line of X. tropicalis was used to examine the process of cornea-lens transdifferentiation in which green fluorescent protein (GFP) is expressed in differentiated lens cells under the control of the Xenopus gamma1-crystallin promoter element. al. It is found in Nigeria, and from Sudan to South Africa where they are plentiful in the rivers and ponds within the southeastern portion of Sub Saharan Africa. The genome of X. tropicalis has been sequenced (Hellsten et al. Reference: EXRC. The cardiac versus skeletal expression patterns of actins are probably sequence-dependent. Xenopus Laevis should be housed at 20-23 degrees C (68-75 degrees F). Room Environmental Parameters. XenOrtho DB (Xenopus Ortholog DataBase) is a database in which ortholog genes of Xenopus tropicalis, Xenopus laevis and humans are listed. Session et al. Xenopus Tropicalis should be housed at 23-28 degrees C (75 -80 degrees F). The African clawed frog Xenopus tropicalis has several characteristics that facilitate studies of developmental and reproductive toxicity. Due to the tetraploid nature of Xenopus laevis, each of these three alpha actin genes is present in at least two copies. Xenopi cannot raise their body temperature by producing metabolic heat. Xenopus laevis (African clawed frog) Status. The African clawed frog (Xenopus laevis, also known as the xenopus, African clawed toad, African claw-toed frog or the platanna) is a species of African aquatic frog of the family Pipidae.Its name is derived from the three short claws on each hind foot, which it uses to tear apart its food. In the present study, membrane integrity and DNA stability of Xenopus laevis and Xenopus tropicalis spermatozoa were evaluated in response to cryopreservation with or without activation, a process that happens upon exposure to water to spermatozoa of some aquatic species. Verlag der Zeitschrift für Naurforschung, Germany Google Scholar. Xenopus laevis has long been a popular model for studies of development and, ... and genetically much more convenient diploid Xenopus tropicalis (Vogel, 1999). Jump to: navigation, search. 1. 2010). Garland Publishing Inc, New York ISBN 0-8153-1896-0. The African Clawed Frog (Xenopus laevis) is also known to tropical fish keeping enthusiasts as the Xenopus, African Clawed Toad, or African Claw Toed frog and is the only amphibian that actually has claws. *Significantly greater or less (p < 0.05) than the corresponding values for controls. Garland Publishing Inc, New York ISBN 0-8153-1896-0. Lateral line systems, usually found in fish, detect vibrations in the water. Procedures input by VGP link to Kroll lab Xenopus environmental procedure SOP document at Washington University animal facility: Modified format below: A. The word Xenopus means "strange foot" and laevis means "smooth". Introduction. A dye exclusion assay revealed that sperm plasma membrane integrity in both species decreased after freezing, more so for … The early development of Xenopus Laevis, an atlas of the histology. Robust and efficient protocols for fertilization and early embryo care of Xenopus laevis and Xenopus tropicalis are essential for experimental success, as well as maintenance and propagation of precious animal stocks. In front of it tropicalis p27 Xic1 promoter driving GFP expression in the neural tube and the.. Development database has provided significant insights into vertebrate evolution and the last common ancestor of.. 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